Tulloch goes by "Bitsie", which is not a contraction of her given name, Elizabeth, but an homage to her oddly nicknamed grandfather, a World War II bomber pilot, who was also involved in the 1961 Goldsboro B-52 crash.

Tulloch graduated magna cum laude from Harvard University with a double major in English and American Literature and Visual and Environmental Studies.

[7] Tulloch's first acting credit was as R2-D2's girlfriend in R2-D2: Beneath the Dome, a mockumentary produced by George Lucas telling the life story of the fictional robot R2-D2 from the Star Wars film series.

The Los Angeles Times wrote, "Superbly performed... the scene at the end of the play, between Sally (Bitsie Tulloch) and Jack (Clark Freeman), was heartbreaking and beautiful...

She was in Lakeview Terrace directed by Neil LaBute, opposite Samuel L. Jackson, Kerry Washington, and Patrick Wilson, and worked with Barbara Hershey on a film called Uncross the Stars.

Tulloch played Marilyn Sitzman, a witness to the Kennedy assassination, in the 2013 film Parkland, opposite Paul Giamatti and Billy Bob Thornton.

In early 2010, she filmed Most Likely to Succeed, a half-hour comedy pilot for Imagine Entertainment through the FOX network, and had a recurring role on NBC's Outlaw.

She played the role of Juliette Silverton / Eve in the fantasy police procedural drama set in a world where characters inspired by Grimms' Fairy Tales exist.

[18] Tulloch and Giuntoli maintained two residences, including a 1920s remodeled Dutch Colonial in Portland and a 1920s Spanish house in Hollywood Hills.

Tulloch was nominated for a 28th Annual LA Weekly Theater Award for her performance in Sam Forman's play Quarterlife (March 2007).