Following a series of cat fights and arguments, the women's lives are interrupted by the arrival of four male death row inmates led by "Crazy Boy" Henderson (Gabriele Tinti), who break into the prison.

The male convicts proceed to rape, mutilate and torture the female inmates (involving a sick game of Russian roulette) and executions.

Henderson and the remaining male cons attempt to break out using the warden (Lorraine De Selle), Emanuelle and a wounded sheriff as human shields.

[3] AllMovie reviewed the film saying: "For even the most jaded fans of the genre, Women's Prison Massacre is as entertaining and arousing as (an) autopsy footage.

As unpleasant as it is, though, the film doesn't begin to approach the depths reached by Mattei's previous effort (Violence in a Women's Prison (1982)) which features the same cast, crew, and basic plot.