The Soviet Union would eventually be dissolved by the resigning Soviet president Mikhail Gorbachev (25 December 1991) and would formally split itself into Belarus, the Russian Federation and Ukraine by signing declarations in Białowieża Forest by its respective presidents (Lukashenko, Yeltsin, Kravchuk) on 26 December 1991.

After the dissolution of Czechoslovakia, Ukraine recognized and re-established diplomatic relations with both the Czech Republic and Slovakia on 1 January 1993.

[2][3] The Embassy promotes the development of good neighborly relations between Ukraine and the Czech Republic at all levels to ensure the harmonious development of mutual relations and cooperation on issues of mutual interest.

[4] The Embassy's priorities also include the care of numerous Ukrainian burial sites in the Czech Republic.

During 2017–2019, the Ministry of Foreign Affairs of Ukraine paid for the lease for the next 10 years and repaired 36 places of memory.