[7] She was accepted into the American Academy of Dramatic Arts shortly after high school but never attended, as it conflicted with a film opportunity.

Hampshire starred alongside Kevin Zegers and Samaire Armstrong in the 2006 romantic comedy It's a Boy/Girl Thing, in which she played the character Chanel.

[10] Hampshire has also done voice-acting, voicing the character Misery on the animated series Ruby Gloom, Diana Barry in Anne of Green Gables: The Animated Series, Chloe Crashman in Carl Squared, Starr in 6Teen, and Alyson Malitski in Braceface.

[11] Also in 2012, Hampshire starred in David Cronenberg's Cosmopolis as Jane Melman,[6][12] and co-starred in Sean Garrity's comedy film My Awkward Sexual Adventure, for which she received positive critical notice.

[17] In late 2015, Hampshire was cast in Xavier Dolan's independent film The Death and Life of John F. Donovan, which also stars Natalie Portman, Kathy Bates, and Susan Sarandon.

[21] In 2006, she married Matthew Smith, a former football player turned agent-in-training at the William Morris talent agency.