[1] He rejoined the Wiesenthal Center in 1986 and uncovered the postwar escape of hundreds of Nazi war criminals to Australia, Canada, Great Britain and other countries.

Since the Fall of Communism and the dissolution of the Soviet Union, Zuroff has played a major role in the efforts to convince Lithuania, Latvia, and Estonia and other post-Communist societies to confront the widespread complicity of their nationals in the crimes of the Holocaust and to prosecute local Nazi collaborators.

His public advocacy on these issues was instrumental in the submission by Lithuania and Latvia of indictments (Aleksandras Lileikis, Kazys Gimžauskas, and Algimantas Dailidė) and/or extradition requests (Konrāds Kalējs and Antanas Gecevičius) against local Holocaust perpetrators.

The country did not prosecute anyone as part of Simon Wiesenthal Center's Operation Last Chance and it also proved to be "technically impossible" to reverse the rehabilitations of Romanian war criminals.

[4][5] In 1993, Zuroff was appointed by then Foreign Minister Shimon Peres to serve on the joint Israeli-Lithuanian commission of inquiry established to deal with pardons issued by the authorities of Lithuania, which had recently regained independence, to people suspected of Nazi war crimes,[6] which has succeeded to date in achieving the cancellation by the Lithuanian authorities of approximately 200 rehabilitations granted to individuals who had participated in the murder of Jews during the Holocaust.

[7][8] According to Zuroff, the Holocaust should not be equated with other tragedies,[9] describing the declaration as "the main manifesto of the false equivalency movement"[10] and stating it is supported by right-wing parties in countries in Eastern Europe.

[12] In 2002, together with Aryeh Rubin, the founder of the Targum Shlishi Foundation of Miami, Florida, Zuroff launched Operation Last Chance, which offers financial rewards for information which will facilitate the prosecution and punishment of Nazi war criminals.

[13] His second book on the hunt for Nazi war criminals, Chasseur de nazis (Paris: Michel-Lafon, 2008), written together with French journalist Alexandre Duyck, continues with the story of the renewed efforts spearheaded by Zuroff to hold Holocaust perpetrators accountable, especially in the wake of the breakup of the Soviet Union and the fall of Communism in Eastern Europe, and focuses on the results achieved by "Operation: Last Chance".

[21] Aribert Heim had reportedly killed "hundreds of inmates at the Mauthausen concentration camp in Austria by injecting gasoline into their hearts and performing surgery and severing organs without anaesthesia" notes Zuroff.

In 1995 and 1996, Zuroff was invited to Rwanda to assist the local authorities in their efforts to bring to justice the perpetrators of the genocide which took place in that country in spring 1994, and he has served as an official advisor to the Rwandan government.

In recognition of his efforts as a Nazi-hunter and Holocaust scholar, Zuroff was nominated by Serbian President Boris Tadić and the members of parliament of the Democratic Party of Serbia as a candidate for the 2008 Nobel Peace Prize.

[23] On January 15, 2010, Zuroff was decorated with the Order of Duke Trpimir by Croatian President Stjepan Mesić for special contributions against historical revisionism and for the reaffirming of antifascist foundations of the modern Republic of Croatia.

[24] On February 16, 2017, the President of Serbia Tomislav Nikolić awarded the gold medal for Merit to Zuroff[25] for "exceptional achievements" and his "selfless dedication to defending the truth about the suffering of Jews, and also Serbs, Roma and other nations during World War II".