Frederiksen was born at Fuglsang, the son of Ditlev Friderichsen (1791–1861), the owner of Nøbbøllegård, and wife Maria Hansen (1811–1901).

He was also introduced to the dairy business by Thomas Riise Segelcke and visited a number of the most innovative agricultural estates across Denmark.

The two brothers constructed the Lolland Sugar Factory in 1872–1874, but it was hit hard by a combination of unfortunate circumstances.

It also turned out to be more difficult than expected to convince local farmers to grow sugar beets for the production.

Frederiksen resided at Svingelgård at Nakskov where he engaged in breeding of barley and sugar beet varieties besides his work as consultant and co-editor of Ugeskrift for Landmænd.

He was a member of the Seed Control Commission (Frøkontrolkommissionen) and co-founded Foreningen af danske landbrugskandidater in 1897 and served on the board until his death.

He was a frequent visitor in the homes of prominent cultural and political figures such as Ditlev Gothard Monrad, Carl Ploug, Niels Laurits Høyen and Constantin Hansen.