[4] Khoo's interest in film eventually led him to study cinematography at the City Art Institute in Sydney, Australia.

In 2006, Khoo executive produced Royston Tan’s second feature 4:30 and was invited to direct for the Jeonju Digital Film Festival in Korea – No Day Off the story of an Indonesian maid.

He produced Royston Tan's 881 a box office hit and received the highest arts honor the Cultural Medallion by the President of Singapore in the same year.

In 2009, Khoo entered into a partnership with Infinite Frameworks to form a new company, Gorylah, specializing in the production of genre films.

Gorylah's maiden effort, Darah, went on to win the Best Actress award at the 2009 Puchon International Fantastic Film Festival.

Centre Pompidou in Paris held an Eric Khoo retrospective in 2010 and he was featured in Phaidon Books, Take 100 - The Future of Film, 100 new directors.

In the Room will also feature a homage to controversial cabaret dancer Rose Chan, the subject of a biopic that was first announced in 2009 but is still under development.

It was initially unable to be screened for commercial release in Singapore as Khoo refused to edit two scenes that exceeded the MDA's film classification guidelines.

[8] Later, the subtly different 'international version' of the film was given a commercial release on 25 February 2016, after it was passed uncut with an R21 rating by the Media Development Authority.

[11] In addition, Khoo released the telemovie Wanton Mee on okto in March, before it travelled to the Culinary Zinema section of the 63rd San Sebastian Film Festival in September and the 10th Culinary Cinema programme of the 2016 Berlinale in February, where chefs like Alexander Dressel took turns serving a menu inspired by the programme's films.

[12] In 2016, Khoo collaborated with some of Southeast Asia's most renowned filmmakers as part of an omnibus project commissioned by National Gallery Singapore, Art Through Our Eyes.

This project brought together – for the first time – filmmakers Joko Anwar (Indonesia), Ho Yuhang (Malaysia), Brilliante Mendoza (Philippines), Apichatpong Weerasethakul (Thailand), and Eric Khoo (Singapore).