Some of her major roles in anime include: Ami Kawashima in Toradora!, Mimori Togo in Yuki Yuna is a Hero, Asseylum Vers Allusia in Aldnoah.Zero, Elizabeth Liones in The Seven Deadly Sins, Kurapika in Hunter × Hunter, Violet in Violet Evergarden, and Yumeko in Kakegurui – Compulsive Gambler, and Shinobu in Demon Slayer: Kimetsu no Yaiba.

In video games, she provides the voice of Kyoko Kirigiri and Kaede Akamatsu in the Danganronpa video game series, Venti in Genshin Impact, Juna Crawford in The Legend of Heroes: Trails of Cold Steel III and IV, Ayesha in Atelier Ayesha: The Alchemist of Dusk, Ann Takamaki in Persona 5, and Raging Bull in Ys IX: Monstrum Nox, Secret of Mana, Final Fantasy VII Remake.

[4] At La Reina High School, she was active in the Speech and Mock Trial team,[5] winning the California state championship in 2008.

[6][11][12] After graduating high school in 2008,[13] she attended California State Polytechnic University, Pomona where she intended to major in graphic design.

[5] On Oliver's suggestion that she was pretty good at it, she changed her major to theatre,[4][16] and continued to take voice acting lessons and workshops while studying at school.

[18][19][20] At one point in college, she had some health issues that led her to take an absence from school and acting, so she pursued production work, which her parents thought would make for a more practical career.

[21] Video game reviewer Sean Madson of Diehardgamefan found the dub to be of decent quality, but said Ayesha "sounded a bit too obnoxious with her airheadedness.

"[22] Matt Sainsbury of Digitally Downloaded thought the dub was reasonable but disliked their American accents as not suiting the Japanese personality of the game.

[5] Her biggest role that year was Ami Kawashima in the romantic comedy Toradora!, a classmate who harbors a mean and bratty personality behind a cheerful perfect-looking facade of a model.

[4] In 2015, Harlacher voiced Mimori Togo in Yuki Yuna is a Hero, one of the main heroines who are called to save their world by interacting in an alternate dimension, where they have super powers.

[21] Anime News Network reviewer Theron Martin said that the cast was "appropriately chosen for their roles in a vocal quality sense and handled the acting capably.

"[34] In the same year, she voiced main character Princess Asseylum Vers Allusia in Aldnoah.Zero as well as Elizabeth in The Seven Deadly Sins, both of which had English dub premieres on Netflix.