In 1963, she became the American representative of Galleria Del Deposito, which featured work by European artists who made functional art objects, such as trays or jewels.

Her gallery showed the work of conceptual artists, including John Baldessari, James Lee Byars, Douglas Huebler, and her daughter, Eugenia P. Butler.

[3] She attended Scripps College and served as a master sergeant in the Marines during World War II where she met her future husband James G. Butler, a lawyer and fighter pilot.

It was at this time that Butler was introduced to Galleria del Deposito, a Genoa based art collective of which Lucio Fontana, Victor Vasarely, and Eugenio Carmi were a part.

Later that same year James Lee Bryars, a conceptual and performance artist, built a block wall separating Butler's office from the gallery.

The most controversial piece to come out of the Eugenia Butler Gallery was Swiss artist Dieter Roth's exhibition, "Staple Cheese (A Race)" (1970).