The Eumenophorinae are a subfamily of tarantula spiders (family Theraphosidae).

They are known from genera distributed across Sub-Saharan Africa, the south of the Arabian peninsula, Madagascar and its associated islands, and parts of India.

[2] At about 158-160 million years ago (Mya), Gondwana split up and the Indo-Madagascan plate drifted away from the rest of the super continent.

In 2005, Richard Gallon described the monotypic genus Mascaraneus, which lacks the stridulatory spike setae.

[2] The monophyly of the Eumenophorinae has been confirmed in a number of molecular phylogenetic studies.