Approximately 2,527 kilometres (1,570 mi) long, it connects Saint Petersburg with Merzifon in north central Turkey.

Between its northern terminus in Russia and its southern end, it passes in addition through Belarus and Ukraine.

Between the ports of Odesa / Chornomorsk on Ukraine's southern coast and ports of Turkey (particularly, Samsun) vehicles are required to cross the Black Sea by ferry over a distance of 731 kilometres (454 mi).

The best alternative is the ferry to Samsun from Chornomorsk, about 2 km (1.2 mi) away from Odesa.

Turkey Russian hard rock band Alisa has a song called "Trassa E-95" [1] dedicated to the road between Moscow and Saint Petersburg (previously part of E95, now part of E105).