Her best result in the Biathlon World Cup is 3rd place in the pursuit in the 2016–17 season.

Her best result here was 3rd place in relay, shared with her Czech teammates Gabriela Soukalová, Jitka Landová and Veronika Vítková.

[3] On 17 March 2022 Puskarčíková announced on her social media end of career.

[4] 1 medals (1 bronze) 1 medal (1 bronze) In 2019–2020, she was married to former Czech biathlete Lukáš Kristejn [de] and competed under name Eva Kristejn Puskarčíková.

On 8 January 2024, Puskarčíková with her partner Thomas Bormolini announced her pregnancy in a post on Instagram.