Everglades University was originally called the American Flyers College when it was founded in Fort Lauderdale, Florida, in 1990.

[9] In January 2003, the Florida Department of Education granted approval to open the Sarasota Branch Campus of Everglades University.

[11] Later that year, the state of Florida Department of Education granted approval to open the Orlando Branch Campus of Everglades University.

[14][15][16] In 2014, Everglades University was granted approval to open the Off-Campus Instructional Site of the Sarasota Branch Campus, in Tampa, Florida.

Everglades University was also granted approval to relocate the Orlando Branch Campus to Maitland, Florida.

[21] Their website and other literature about the university stipulate that they have small class sizes, with students who predominantly want to be educated while learning and/or adult learners.

This approach is aimed to focus students' learning on an individual subject at one time when compared to traditional university models, where numerous modules are studied together.

The university building is 37,000 square feet and includes offices, classrooms, computer labs, a library and student lounge.

The campus is known for its fundraising efforts, with staff and students previously participating in a Walk to Cure Arthritis, Making Strides Against Breast Cancer Walk, collecting toys for Toys for Tots, and hosted several blood drives with OneBlood.

[24] The Orlando Branch Campus of Everglades University is located in a 28,000 square-foot building on Trafalgar Court, in Maitland, Florida.

The staff, faculty, and students at the Boca Raton Campus have participated in the Walk to Cure Arthritis, Making Strides Against Breast Cancer Walk, collecting toys for Toys for Tots, and hosted several blood drives with OneBlood.

The Tampa off-campus instructional site offers a library, a computer lab, student lounge, bookstore, 17 well-lit classrooms, three laboratories, and 11 offices.

The campus is close to some of the area's attractions, such as Busch Gardens, The Lowry Park Zoo, Historic Ybor City, and Downtown Tampa.

Local industry professionals from such companies as Duke Energy, H. Lee Moffitt Cancer Center, Greenroots Nutrition, and Tampa Fire Rescue attend advisory meetings three times a year.

This includes community service events and initiatives such as a Help for Haiti Relief Donation Drive to send vital supplies, fundraising activities to support the Cystic Fibrosis Foundation, and a drive to collect goods for Pet Rescue organizations in South Florida.