At that time, women's football in the Netherlands was amateur, and most talented female players left for Germany and other countries with professional leagues.

[5] The club played its first official match on 29 August 2007, also the first-ever Eredivisie game, home at the Arke Stadion in front of 5.500 spectators, losing 2–3 against SC Heerenveen.

In the Dutch Cup, the KNVB decided to take all Eredivisie clubs out of the competition in the Round of 16, in order to have the professional players prepared for the UEFA Women's Euro 2009.

It reached the semifinals of the Dutch Cup, where it lost to Ter Leede in a penalty shoot-out after a 3–3 draw.

The club brought in American players Ashley Nick and Caitlin Farrell, new goalkeeper Sari van Veenendaal, and striker Joyce Mijnheer [nl].

The team clinched its first title on the last matchday, in a home match at the Grolsch Veste in front of over 7.000 spectators, a 4–1 win over Willem II, ending AZ Alkmaar's three-year championship run.

In the second half of the season, ADO Den Haag extended its lead, and Twente finished in second place, 14 points back.

With the departures of Ashley Nick, Blakely Mattern, Joyce Mijnheer, and Lorca Van De Putte before the season and Courtney Goodson in the winter break, the team brought in Sherida Spitse and Jill Roord.

[8] The Tukkers narrowly missed a double, losing the Dutch Cup final on penalties to ADO Den Haag.

The club won the Dutch championship for the fourth consecutive year after a 3–0 win over PEC Zwolle on 20 May 2016.

[21] FC Twente returned to the top of the league in the 2018–19 season, earning is sixth national championship.

Each team won on the road, with Twente winning in St. Pölten 4–2, with new striker Fenna Kalma scoring a hat trick.

[28] In the Dutch Cup, Twente won its only match, defeating lower level club Nooit Gedacht of Geffen 12–0 in the round of 16.

After the first half of the season, Twente was in first place by one point over PSV, which along with Ajax were clear top three teams in the league, based on results.

[30] After the championship playoff, Twente again held a one point lead over PSV, and the two teams qualified for the 2021–22 UEFA Women's Champions League.

[31] Twente clinched the title in the penultimate week, defeating the weaker ADO Den Haag as Ajax bested PSV.

Twente had three of the four top goal scorers in the league, in Renate Jansen, Fenna Kalma, and Anna–Lena Stolze, respectively.

However, Twente lost to Benfica in the second round, with the decisive match being a 4–0 loss in the second leg in Seixal, Portugal.

[39] Twente also lost early in the KNVB Women's Cup, losing in the quarterfinals 3–0 to Feyenoord in Rotterdam.

[47] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.