He was father of Vittoria della Rovere, Grand Duchess of Tuscany.

His father Francesco Maria II della Rovere was popularly urged to remarry after the death of his first wife in 1598, with the marriage producing no heirs.

This same year, in order to produce an heir himself, he married Claudia de' Medici, daughter of Ferdinando I de' Medici, Grand Duke of Tuscany and Christina of Lorraine.

The following year, Claudia gave birth to a daughter Vittoria della Rovere.

[2] He was to be in the fold of enlightened absolutism upheld since Francesco Maria I della Rovere.