Epidemics carried away many in 1813, but the numbers continued to swell as revival meetings won numerous converts,[2]: 403  and while the new building was complete by 1814, an expansion was necessary as early as 1821.

[1] The earliest members of the Greek Orthodox faith resident in Steubenville had no church building; some were members of the Holy Trinity parish, which worshipped on the upper floors of commercial buildings, while others crossed the Ohio River to Weirton for worship,[3] even though both the Fort Steuben Bridge and the Market Street Bridge charged tolls at the time.

[3] Holy Trinity is a composite Neoclassical building; the walls are brick with significant sandstone elements, covered by an asphalt roof and resting on a stone foundation.

[1] The main entrance is set in a recessed portico at the center of the facade, accessed by a small flight of steps and sheltered by a colonnade of four Ionic columns.

A tall square tower, once topped with a cupola, is placed along the South Street side, while a far larger dome covers the central portion of the building.