It is a subsidiary of First National of Nebraska, Inc., a bank holding company primarily owned by the Lauritzen family.

Attached to a 420-room hotel and a 550-stall parking garage, it became one of the most modern buildings in the region, providing economic development in downtown Omaha.

[5] In 2000, First National Bank designated two parcels of green space for the city of Omaha.

They are the current sites of two sculpture parks called "Spirit of Nebraska's Wilderness" and "Pioneer Courage".

[13] In 2018, the bank ended its credit card affinity program with the National Rifle Association of America (NRA) due to customer complaints after the Parkland high school shooting.

[15] Notable office locations of FNBO include: In August 2016, First National Bank of Omaha was disciplined for numerous deceptive marketing and unfair billing practices regarding credit card add-on products such as credit monitoring, identity theft monitoring, and debt relief products and services that they did not receive.

At the same time, Marquette Bank Minneapolis sued FNBO for charging customers in Minnesota interest rates that were higher than the 12% legal limit in that state.