Zygaena trifolii, the five-spot burnet, is a day-flying moth in the family Zygaenidae found in North Africa and Europe.

It was described by the German zoologist Eugenius Johann Christoph Esper in 1783 from the type specimen found in Frankfurt am Main, Germany.

Pupation of subsp descreta takes place in June and July in a cocoon which is spun high on marsh vegetation such as soft rush (Juncus effusus).

Pupation of subsp palustrella takes place close to the ground and concealed amongst grass and other herbage in late-April to May.

[2][3] It is found in North Africa (Algeria, Morocco and Tunisia through the western Mediterranean, Great Britain and central Europe to Ukraine.