Coping with chauvinistic bartender Earl (Geoffrey Lewis) and the greedy and obnoxious banker Farley (Jim B. Baker) who holds the mortgage, as well as her mother Velma (Sudie Bond) and straight-laced sister Fran (Lucy Lee Flippin), causes most of the conflict in the series.

The rest of Flo's staff at the Yellow Rose includes her childhood best friend Miriam (Joyce Bulifant) as waitress/bookkeeper, and chain-smoking piano player Les (Stephen Keep).

Randy, the mechanic (Leo Burmester) who worked at the garage next door, and Chester (Mickey Jones), were regular customers.

The theme song, "Flo's Yellow Rose", written by Fred Werner and Susie Glickman, was sung by Hoyt Axton who would co-star with Holliday in the 1984 film Gremlins.

Flo was broadcast in these following timeslots during its two-season run on CBS: The short first season (spring 1980) was a ratings success ranking at No.