Flora Perini (20 November 1887 – 23 September 1975) was an Italian operatic mezzo-soprano who had a prominent opera career in Europe, South America, and the United States during the first half of the twentieth century.

She studied at the Accademia Nazionale di Santa Cecilia before making her professional opera début at La Scala in 1908 as Anacoana in Franchetti's Cristoforo Colombo.

Over the next several years she appeared in operas in Nice, Venice, Trieste, Turin, Bologna, Madrid, Barcelona, Saint Petersburg, Rio de Janeiro and Montevideo.

[2] In 1915, Perini made her American début at the Metropolitan Opera as Lola in Mascagni's Cavalleria rusticana with Margarete Matzenauer as Santuzza, Luca Botta as Turiddu, and Gaetano Bavagnoli conducting.

[2] Perini can be heard singing the role of Maddalena in the famous 1917 Victor recording of the Rigoletto quartet with Enrico Caruso, Amelita Galli-Curci and Giuseppe De Luca.