Florencia studied at the Colegio Nuestra Señora de la Misericordia in Recoleta, Buenos Aires, Argentina.

In late 2008, it was announced that Florencia Bertotti would return to television with a new telenovela targeted to children, which would be produced by her and her husband Guido Kaczka.

In 2014, she was the protagonist of the television series Guapas with Mercedes Morán, Araceli González, Carla Peterson and Isabel Macedo.

In 2016 Florencia Bertotti returns to television starring in the series Silencios de familia with Adrián Suar and Julieta Díaz.

In 2019, she was part of the play 100 metros cuadrados with María Valenzuela and Stéfano de Gregorio directed by Manuel González Gil.

On 15 March 2021, Florencia Bertotti launched a new space, which she called Pancha Recreo, this section contains reclaimed wood games, without batteries, without rules and without instructions to accompany the little ones in their different stages of development and growth.

In this new section there is also a great variety of books for children of different ages, which were specially chosen and previously read by Florencia Bertotti.