Along the way it runs between a pair of churches before the driveway to the Cracker Creek Campground, which also leads to the NRHP-registered Gamble Place Historic District.

Dunlawton Avenue takes SR 421 in a north-northeasterly direction, then curves more towards the east just before the intersection with County Road 483 (Clyde Morris Boulevard).

From there it runs east-northeast, flanked by medical offices, shopping centers, and apartment buildings, many of which are obstructed by native foliage.

[5] Most of the shopping centers continue to remain hidden by foliage even as the route approaches the intersection with Florida State Road 5A, with the exception of the one on the southeast corner of SRs 5A and 421.

Standard commercial development gives way to medical and legal offices, as well as residencies, with the solitary exception of a boat dealership, which is where it takes a bend to the east-southeast before the crossing of the main line of the Florida East Coast Railway, then passes by the Port Orange Elementary School, just before running along the northern edge of the Dunlawton Avenue Historic District.