Fly Tiwi is an Australian airline based in Darwin, Northern Territory, offering scheduled passenger services between the Northern Territory capital and communities located on the Tiwi, South Goulburn and Croker islands, as well as a number of remote Arnhem Land communities.

In January 2009 a third route was added providing daily services to South Goulburn Island and the community of Minjilang on Croker Island, and in May began services to Ramingining three times per week, adding a fifth route to Gapuwiyak twice per week in early 2010.

[3] In February 2012, Fly Tiwi was one of a number of aviation companies that participated with the Northern Territory Police in 'Operation Bristol', a crackdown on the trafficking of illegal drugs to remote communities which resulted in the seizure of cannabis and kava with an estimated street value of over $300,000.

Typically, twin piston engine, 10 seat Cessna 402 and 404 types are used for regular services.

The fleet also includes pressurised Fairchild Metro and Cessna 441s, used for longer routes, as well as a number of smaller types that can operate charters for Fly Tiwi.