Fort Niobrara National Wildlife Refuge is located in the U.S. state of Nebraska and includes 19,131 acres (77.42 km2).

From 1879 to 1906, the Fort Niobrara Military Reservation was located on what later became refuge lands to house a garrison of the U.S. Cavalry.

Embracing parts of townships thirty-three and thirty-four north, ranges twenty-six and twenty-seven west, Sixth Principal Meridian, Nebraska, the same being a part of the abandoned Fort Niobrara Military Reservation.

This unusual alteration to the otherwise relatively featureless Great Plains presents a unique habitat that fosters numerous plant and animal species.

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