Fosmids have high structural stability and have been found to maintain human DNA effectively even after 100 generations of bacterial growth.
[3] The fertility plasmid or F-plasmid was discovered by Esther Lederberg and encodes information for the biosynthesis of sex pilus to aid in bacterial conjugation.
There are now many companies that can create a fosmid library from any sample of DNA in a very short period of time at a relatively low cost.
[8] The fosmid system is excellent for rapidly creating chromosome-specific mini-BAC libraries from flow-sorted chromosomal DNA.
The major advantage of Fosmids over other cosmid systems lies in its capability of stably propagating human DNA fragments.
It has been found that the stability increases dramatically when the human DNA inserts are present in single copies in recombination deficient E. coli cells.