He was counsellor to the Parlement of Brittany and advocate general to the Grand Conseil.
In 1596, Henry IV named him maître des requêtes, and then, in 1604, Conseiller d'État.
François d'Amboise became seigneur of Vezeul, Bourot, Neuillé-le Lierre, Brouard, Lespinière, La Huardière in Touraine, of Houvoy, Malnoue, Courserin, Plessis-Bourré, Hémery, and Baron of La Chartre-sur-le-Loir.
His son was Antoine d'Amboise, seigneur of Clos Lucé, colonel of the regiment of Amboise, maréchal de camp et governor of the citadel of Trin (Piedmont).
François d'Amboise wrote a comedy in verse entitled the Néapolitaines (1584) and several works of poetry, including an elegy on the death of Anne de Montmorency, Élégie sur le trépas d'Anne de Montmorency (1568).