Then he continue with other great directors such as Elio De Capitani, Mario Missiroli, Luca Ronconi, Giuseppe Patroni Griffi and many more.

In film, Acquaroli debuted in Diaz – Don't Clean Up This Blood by Daniele Vicari, followed by Oranges & Hammer by Diego Bianchi, Pasolini by Abel Ferrara, Era d'estate by Fiorella Infascelli, Mia Madre by Nanni Moretti, I Can Quit Whenever I Want by Sydney Sibilia, The Last Will Be the Last by Massimiliano Bruno, "Dogman" by Matteo garrone, "The Best Years" by Gabriele Muccino, "My Name Is Mohammed" by Pascaljevich, and Adults in the Room by Costa-Gavras.

Acquaroli is the narrator in "I mille giorni di Mafia Capitale", a documentary aired on Rai 3 in September 2017.

Acquaroli portrays Ebal Violante in the fourth season of American television series Fargo.

[1] Since 2018, Acquaroli has supported Médecins Sans Frontières; he has lent his voice to the video and radio spot of the campaign "Cure in the heart of the conflicts".