The exhibitions and programs present a broad range of recent developments and aesthetic concerns and have included more than 1,000 artists to date.

The Gallery's program of exhibitions, lectures, film and video screenings, and performances features interdisciplinary and experimental practices.

Exhibitions in 2013 have included: It's the Political Economy, Stupid, The Program, Whisper Down the Lane, and I THINK WE'RE READY TO GO TO THE NEXT SEQUENCE: THE LEGACY OF HALFLIFERS.

Exhibitions from years past have included: Focus Pull: Onion City Off Screen, Frau Fiber: Knock Off Enterprises - the labor behind the label, Justin Cooper: Thread, Asma Kazmi: Relation-Chute, Carla Arocha: Orchid, Andy Roche: Black Iron Vatican, Leif Elggren and Carl Michael von Hausswolff: Inauguration of the Consulate General of the Kingdoms of Elgaland-Vargaland, An Atlas, I Am Eyebeam, Carol Jackson, Feel Tank Chicago: Pathogeographies (Or, Other People's Baggage), Amanda Browder and Stuart Keeler: Urban Warp / Weft sculptural research project, Captive Audience, John Arndt : Empire, Jeanne Dunning: Tomato Fight, Carter: Drawings and Polaroids.

[3] Throughout its history Gallery 400 has commissioned large-scale new works through residencies, comprising varied combinations of workshops for students, public lectures, the creation of large-scale new works, and collaborations between the artist, students, and partner community groups.