[1][2][3] Initially, the regiment was divided into ten companies of men of different national heritage: three German, three Hungarian, one Swiss, one Italian, one French, one Portuguese and Spanish.

In October, 1864, a new Company D, mustered mostly from Malone, New York joined the regiment for one year.

The regiment left the New York State May 28, 1861; served at and near Washington, D. C., from June 1, 1861; in the 1st Brigade, 5th Division, Army of Northeastern Virginia, from July 13, 1861; in Blenker's Brigade, Division of Potomac, from August 4, 1861; in Stahel's Brigade, Blenker's Division, Army of the Potomac, from October 15, 1861; in 1st Brigade, same division, Mountain Department, from April, 1862; in White's Brigade, Army of Virginia, at Winchester, Va., from July, 1862; at Harper's Ferry, W. Va., from September, 1862; at Camp Douglass, Chicago, Ill., from September 27, 1862; near Washington, D.C., 1st Brigade, Casey's Division, defenses of Washington, from December, 1862; in January, 1863, in 3d Brigade, Casey's, later Abercrombie's Division, 22d Corps; in 3d Brigade, 3d Division, 2d Corps, Army of the Potomac, from June 25, 1863; in the 3d, and for a time in the Consolidated, Brigade, 1st Division, 2d Corps, Army of the Potomac, from March, 1864; and was honorably discharged and mustered out, under Col. Augustus Funk, July I, 1865, except (new) Company D, which had been mustered out, June 7, 1865, at Alexandria, Va.[6] During its period of service, 5 officers and 62 enlisted men were killed in action; 3 officers and 49 enlisted men died of wounds received in action; 1 officer and 158 enlisted men died of disease and other causes.

[1] The regiment's uniform was based on that of the 'Bersagliere[note 1] troop of marksmen from the Royal Sardinian Army.

They wore the distinctive black, brimmed hats, called vaira, (intended to defend the head from sabre blows) decorated with dark cock feathers.