Genesee Depot is a small unincorporated community in Waukesha County, Wisconsin, United States.

Genesee Depot was founded mainly by German, Welsh, Irish, and English immigrants in the late 19th century.

This station building was relocated slightly south of where it originally stood on Hwy 83 and currently is Mama D's Coffee shop.

The original town hall, now the Wales-Genesee Lions and Lioness Club, was built in 1912 and is on the National Register of Historic Places.

Lunt and Fontanne's residence in Genesee Depot was their summer home and a popular gathering place for theater people.

Named after John Magee, who helped bring the railroad through Genesee Depot, the school was completed and opened in 1922.