Gertrude of Babenberg (Czech: Gertruda Babenberská; c. 1118 – 8 April 1150), a member of the House of Babenberg, was Duchess consort of Bohemia from 1140 until her death, by her marriage to the Přemyslid duke Vladislaus II.

[1] She was the daughter of Margrave Leopold III of Austria (died 1136) and his second wife, the Salian heiress Agnes of Waiblingen.

Upon the death of her father, Gertrude's brother Leopold IV succeeded as Austrian margrave and in 1138 married the Přemyslid princess Maria of Bohemia, a cousin of her future husband.

Through her mother, Gertrude was a half-sister of the Hohenstaufen king Conrad III of Germany, thus a good catch for Vladislaus.

At the time of the siege of Prague by his cousin Count Conrad II of Znojmo in 1142, she successfully defended Prague Castle with the help of her brother-in-law Děpold, while Vladislaus sought assistance from King Conrad III.