Segura lost the bout by unanimous decision, but subsequently claimed the interim title by knocking out Canchila in a rematch on March 14, 2009.
[2][3] Segura defended his newly acquired title on July 25, 2009 by stopping Juanito Rubillar in six rounds.
[4] On August 28, 2010 Giovani fought undefeated WBO Light Flyweight champion Iván Calderón in a unification bout.
[5][6] Segura went on to knock out Calderón in the eighth round, successfully unifying his WBA title with the Puerto Rican's WBO and Lineal Light Flyweight Championships.
[10] Following that victory, Segura vacated his titles in order to move up to the Flyweight division where he was KO by Brian Viloria.