Gladys Lake Ecological Reserve is an ecological reserve located in the Eaglenest Range of the Spatsizi Plateau in north-central British Columbia, Canada.

It was established in 1975 under the Ecological Reserves Act to facilitate scientific research of the region's alpine-subalpine ecosystems while discouraging outdoor recreation use.

[3] The reserve is an enclave within the larger Spatsizi Plateau Wilderness Provincial Park, though with stricter protections and limited public access.

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