The optical axes of the calcite crystals are parallel and aligned perpendicular to the plane of reflection.
Birefringence splits light entering the prism into two rays, experiencing different refractive indices; the p-polarized ordinary ray is totally internally reflected from the calcite–cement interface, leaving the s-polarized extraordinary ray to be transmitted.
The prism can therefore be used as a polarizing beam splitter.
Traditionally Canada balsam was used as the cement in assembling these prisms, but this has largely been replaced by synthetic polymers.
[1] Compared to the similar Glan–Foucault prism, the Glan–Thompson has a wider acceptance angle, but a much lower limit of maximal irradiance (due to optical damage limitations of the cement layer).