Founded in 1957, the club competes in Australia's second-tier National Premier League, with matches played at KGV Park, a 2,000 capacity stadium.

The club was founded in 1957 as Glenorchy Croatia, and experienced almost immediate success, winning the state title for the first time in 1963.

In 2015 Glenorchy Knights were unsuccessful in their application to remain within the NPL Tasmania and returned to lower Southern Championship in 2016.

Glenorchy Knights responded by winning three consecutive Southern Championships in 2016, 2017 and 2018, losing just three time in 49 games.

In 2024, the club completed the double winning the Tasmanian National Premier League and the Lakoseljac Cup under the guidance of James Sherman in his final season as Head Coach.