The edifice was designed in the Lombardic Romanesque Style, with the chapel's hundred-foot bell tower and its cloister, a porch on the east side of the building.

The interior design also follows Romanesque motifs with the incorporation of the ribbed ceilings, arched woodwork, and stained glass.

[1] The organ, located on the left side of the chancel, was modernized by removing cherry stained wood decorations and panels in front of case pipes.

[2] Tommaso Juglaris, an Italian born artist collaborated in the design and creation of the stained glass windows.

The opposite window depicts St. John the Evangelist and was built in memory of Tufts' first president Hosea Ballou II.

When the Crane Theological School closed in 1969, a wing of the chapel was converted to house the new Office of the University Chaplain.