The submarine was built at the Forges et Chantiers de la Gironde shipyard, in Bordeaux, between 1925 and 1927, and commissioned into the Hellenic Navy on 8 June 1928.

After the German invasion of April 1941, together with the rest of the fleet, Katsonis fled to the Middle East, from where she would operate during the next years, with the British pennant number N 16.

During these patrols, Katsonis ambushed and sank an Italian minelayer in the port of Gytheio on 2 April 1943, the Spanish 535-ton merchant vessel San Isidoro off Kythnos three days later, and the freighter Rigel near Skiathos on 29 May.

However, Lt Elias Tsoukalas, the ship's XO, and petty officers Antonios Antoniou and Anastasios Tsigros, managed to swim for 9 hours and reach Skiathos.

[5] In all, Katsonis' career in World War II encompassed seven patrols and four ships sunk:[6] In July 2016 the wreck was located by Greek's Navy Oceanographic vessel HS Naftilos A-478 (Υ/Γ-Ω/Κ Ναυτίλος).