The waters of Guadalupe Canyon are tributary to the San Bernardino River joining it at its mouth at 31°13′33″N 109°16′10″W / 31.22583°N 109.26944°W / 31.22583; -109.26944 just below Dieciocho de Augusto, Sonora.
[4] Other birds in the area are the broad-billed hummingbird, northern beardless tyrannulet, summer tanager, thick-billed kingbird, Elf Owl, black-tailed gnatcatcher, varied bunting, and wild turkey.
[6] Guadalupe canyon is also a wildlife corridor for Mexican gray wolves, and endangered jaguars, whose range bridges between the borders of both countries.
[8] Trees in the riparian habitat areas within the canyon include oak, Arizona sycamore, willow, and Freemont Cottonwood.
[5] In 2020, portions of the canyon's sides were dynamited to make way for construction of the Mexico–United States border wall, prompting heavy criticism.