The street contains the rear entrance to Goodenough College, an international residential centre for postgraduates studying or training in London.

Its restaurant, which was originally named after the architect but is now called Neptune, is said to be almost identical to the RMS Titanic's dining room, which he designed.

[3] Wing Commander F. F. E. Yeo-Thomas GC, MC & Bar (1902-1964), an SOE agent during the Second World War, known by the Gestapo as 'The White Rabbit', lived on Guilford Street.

Guilford Street is the home of Arthur Rowe, the protagonist in Graham Greene's novel "The Ministry of Fear."

The house on Guilford Street is where he administers a mercy killing of his ill wife, survives the blitz, and stores a very unusual cake that is central to the plot of this spy thriller.