The company owns railroads carrying a wide variety of commodities, generating around 40,000 annual carloads over approximately 225 miles of track.

[13] On February 1, 2010, the lease on the Morehead & South Fork was acquired by the Carolina Coastal Railway and was no longer operated by Gulf & Ohio.

[14] Knoxville became the center of Gulf & Ohio operations, with a locomotive shop, corporate headquarters, and special tourist excursions all based in the city.

The company continued to acquire and sell subsidiary railroads, with its presence eventually contained to three states; Alabama, North Carolina, and Tennessee.

[15] Gulf & Ohio extended its presence into South Carolina in November 2010 with the acquisition of the Lancaster and Chester Railway, an independent railroad.

However the early railroads shared a bright red paint scheme and distinctive large white unit numbers applied near the end of the long hood.

[22] Further development of the paint scheme brought about a common logo in the form of a simple black circular herald with a yellow border.