He has worked with artists including Pink Floyd, Roxy Music, Gary Moore, Madonna, Peter Cetera, Michael Jackson,[1] the Smiths, Robert Palmer, Echo & the Bunnymen, Tears for Fears, Icehouse, Bananarama, Iggy Pop, Tom Jones, Debbie Harry, Whitesnake, Womack & Womack, Kirsty MacColl, Coverdale•Page, Lemon Jelly, the Orb, All Saints, Stephen Duffy, Robbie Robertson and A. R. Rahman.

Pratt has also been an actor and worked on TV and film soundtracks, including Dick Tracy (1990), Last Action Hero (1993), Hackers (1995), Still Crazy (1998) and Johnny English Reborn (2011).

[3] In 1986, the Smiths' bassist Andy Rourke was arrested on drug possession charges shortly before the band was scheduled to leave for its North American tour in support of The Queen Is Dead.

Expecting that Rourke would thus be denied work visas for the U.S. and Canada, the band's guitarist, Johnny Marr, asked Pratt to step in.

Producer and keyboardist Patrick Leonard approached Pratt in 1988 about forming a band, and helping him recruit members of what would become Toy Matinee.

[9] He produced, co-wrote and played bass, guitar and keyboards on the music for the 1998 Channel 4 drama series The Young Person's Guide to Becoming a Rock Star.

Pratt also acted in Linda Green and appeared in an episode of the remake of Randall & Hopkirk, starring Vic Reeves and Bob Mortimer.

[13] Pratt's one-man music and comedy show, My Bass & Other Animals, debuted in August 2005 at the Edinburgh Festival Fringe; this led to his book of the same name, published in May 2007.

[14][15] Pratt spent 2011 performing stand-up in Switzerland, Australia, and at the Edinburgh Fringe, as well as on a South American tour playing bass guitar for Dominic Miller.

In April 2010, Pratt joined the Argentine cover band the End Pink Floyd, with Durga McBroom and Jon Carin, in Buenos Aires, Argentina.

Pratt joined the Australian Pink Floyd Show on stage on 13 June 2011 for the Hampton Court Palace Festival for the song "Run Like Hell".

In 2018, Pratt and others formed a new band, Nick Mason's Saucerful of Secrets, to perform Pink Floyd's early psychedelic material.