[2] It is known only from a single adult male specimen collected in a "sawgrass-mangrove area" along the Snake Bight Trail north of Flamingo in Everglades National Park, Florida on 27 August 1965.

[4] Gymnochthebius seminole is a species within the family Hydraenidae, commonly known as minute moss beetles,[5] belonging to the broader order Coleoptera.

[6] Gymnochthebius seminole is a small aquatic beetle that, like other species in the Hydraenidae family, inhabits in moss or accumulations of moist/wet dead leaves and sticks/twigs along the margins of streams, rivers, sinkholes, pools and ponds; also in littoral zone substrata.

[7] Gymnochthebius seminole, like many other species in the family Hydraenidae, has an aquatic life stage where both larvae and adults feed by grazing on surfaces in their freshwater habitats.

[5][7] The larvae play a crucial role in this ecosystem, by catabolism of organic matter by consuming algae and bacteria, they help control the growth of these microorganisms and contribute to nutrient cycling within their environment.