She served during the Second World War, and was adopted by the civil community of Mitcham, Surrey following a successful Warship Week National Savings campaign held in February 1942.

The engines produced a total of 2,000 shaft horsepower (1,500 kW) and gave a maximum speed of 16.5 knots (30.6 km/h; 19.0 mph).

They carried a maximum of 660 long tons (671 t) of fuel oil that gave them a range of 5,000 nautical miles (9,300 km; 5,800 mi) at 10 knots (19 km/h; 12 mph).

For these operations she was detached from the flotilla and carried out these duties in mid July in company with her sisters Fly, Espiegle and Cadmus.

These duties included mine clearance of positions to be used by Allied warships for bombardments prior to military advances.

She carried out these duties into December, when she was moved to the Anzio area to support Operation Shingle, the planned allied landings.

Further work in the Aegean Sea followed, where Mutine suffered slight damage while sweeping one of the new German 'Oboe' mines.

In January 1945 Mutine returned to Malta with Fly and then moved on to resume mine clearance off the Greek mainland with the rest of her Flotilla.

After this requirement ended, Mutine was used as a ship target for aircraft training and returned to the reserve at Chatham.