Thule served in the Far East for much of her wartime career, where she sank thirteen junks, two lighters and five sampans with gunfire in the Strait of Malacca in a twelve-day period between 17 December 1944 to 29 December 1944.

She went on to sink a further five sailing vessels and three coasters, as well as laying a number of mines.

[1] On 18 November 1960, Thule, a member of the 5th Submarine Squadron, was taking part in an anti-submarine exercise off Portland Bill, when she was accidentally rammed by the Royal Fleet Auxiliary tanker Black Ranger when at periscope depth.

Thule's snort was broken, one of her periscopes was bent and her casing was damaged.

[4] Her first commander, Alastair Mars, wrote HMS Thule Intercepts, about her operations from commissioning in Scotland to the end of the war in Australia.