HMS Upshot was a V-class submarine of the Royal Navy.

She was built during the Second World War as part of the second batch (18 in number) of V-class submarines ordered on 21 May 1942.

She was built by Vickers-Armstrong (Barrow-in-Furness), being laid down on 3 May 1943, launched on 24 March 1944, and finally commissioned on 15 May 1944.

The submarine was decommissioned on 2 November 1949 and broken up for scrap in Preston.