The Island Knights supported the Naval Air Ambulance Detachment from Camp Buering, Kuwait & Basra, Iraq from Fall 2005 to May 2012, saving a total of 2,923 lives.

As of August 2019, HSC-25's homeguard unit (stationed at Andersen AFB, GU), has a total of 617 lives saved during Search and Rescue missions, the first being June 27, 1984 with HC-5.

As part of Helicopter Sea Combat Wing, Pacific, HSC-25 is the Navy's only forward deployed Expeditionary Sea Combat squadron, providing Anti-Surface Warfare, Personnel Recovery, Special Operations support, Vertical Replenishment, Search and Rescue, and Medical Evacuation capabilities to ships and units in the Seventh Fleet Area of Responsibility.

At homeguard, HSC-25 maintains an aggressive tactics program in support of both deployed and local special warfare units, Expeditionary and Carrier Strike Groups, as well as providing 24-hour SAR and MEDEVAC services for Guam and the Northern Mariana Islands.

HSC-25 is the first and only forward-deployed vertical replenishment (VERTREP) squadron in the Navy and is tasked with supporting Seventh Fleet units in the Western Pacific, Indian Ocean, North Arabian Sea, and Persian Gulf.

In the Fall 2005, HSC-25 was again the first HSC Squadron to assume a new mission, providing essential Air Ambulance services for Coalition Forces, and Compotent Command (CFLCC) in Camp Buering, Kuwait.

HSC-25 also conducts airborne firefighting utilizing externally carried buckets, Vertical Onboard Delivery (VOD), drone and torpedo recovery, special operations airborne support, and fleet logistics support for all military activities in the Guam area, including the Maritime Prepositioned Ships (MPS) operating in the local area.

[citation needed] The trident held by the eagle symbolizes the emphasis being placed on the combat capabilities of the helicopter in the maritime environment as well as pays homage to the squadrons naval heritage.