However, the absence of E-V68* and E-V257* in the Middle East makes a maritime spread between northern Africa and southern Europe a more plausible hypothesis.PF2431 is the sister branch of M81 which was discovered in Paolo Francalacci (2011).
This mutation has been discovered in North Africa (in Souss in Morocco, in central and eastern Algeria, West Nile in Egypt), the Sahel (Chad, Gambia), Western Europe (United Kingdom (Derbyshire), Germany, Switzerland, Spain, Italy) and Near Eastern (Turkey, Karabakh and Urmia).
Archeology unearthed the remains of a member of the Hungarian conquering elite was analyzed from branch E-FGC19010, it had been discovered in Sandorfalva in Hungary and is dated to the second half of the tenth century.
[14] A skeleton was discovered at the Monastery of San Pietro, Villa Magna in Italy, whose DNA belongs to the same branch and lived around 1180CE.
[15] Scientists have examined the DNA of a mass grave of victims of the bubonic plague in Ellwangen in Germany, this one dates from the 16th century and belongs to another branch E-FGC18981.
In Tunisia, it reached 100% frequency among a sample of Arabs from Zriba,[24] 89.5% in Andalusians (Qalaat-al-Andalous) and 100% in Berbers from Chenini-Douiret, Jradou and Takrouna.
At the eastern extreme of this core range,[23] M81 is found in 28.6% (10 out of 35 men) in El-Hayez in the Western desert in Egypt The pattern of distribution and variance to be consistent with the hypothesis of a post Paleolithic "demic diffusion" from the Middle East.
[3] The ancestral lineage of E-M81 in their hypothesis could have been linked with the spread of Neolithic food-producing technologies from the Fertile Crescent via the Nile, although pastoralism rather than agriculture.
E-M81 and possibly proto-Afroasiatic language may have been carried either all the way from Asia, or they may represent a "local contribution to the North African Neolithic transition".
The E-M81 subclade has been found in ancient Guanche (Bimbapes) fossils excavated in Punta Azul, El Hierro, Canary Islands, which are dated to the 10th century (~44%).
[33] E-M81 is also found in other parts of Europe, such as Britain – especially Wales and Scotland – and France, where it has an overall incidence of 2.7% (15/555), with frequencies surpassing 5.0% in Auvergne (5/89) and Île-de-France (5/91).
In a 2014 study by Stefania Sarno et al. with 326 samples from Cosenza, Reggio Calabria, Lecce and five Sicilian provinces, E-M81 shows an average frequency of 1.53%, but the typical Maghrebin core haplotype 13-14-30-24-9-11-13 has been found in only two out of the five E-M81 individuals.
These results, along with the negligible contribution from North-African populations revealed by the admixture-like plot analysis, suggest only a marginal impact of trans-Mediterranean gene flows on the current SSI genetic pool.
[39][41] As a result of Spanish and Portuguese colonization of the Americas, this sub-clade is found throughout Latin America, for example 6.1% in Cuba, (8 out of 132),[42] 5.4% in Brazil (Rio de Janeiro) (6 out of 112), "The presence of chromosomes of North African origin (E3b1b-M81;[26] can also be explained by a Portuguese-mediated influx, since this haplogroup reaches a frequency of 5.6% in Portugal,[30] quite similar to the frequency found in Rio de Janeiro (5.4%) among European contributors.
The following gives a summary of most of the studies which specifically tested for E-M81, showing where its distribution is greater than 1% in Europe, North Africa, the Middle East and Latin America.
The remaining two are significantly smaller, and include scattered individuals in Germany, Spain, Latin America, Egypt, and Ethiopia.
Without information about M293 in the Maasai, Hema, and other populations in Kenya, Sudan, and Ethiopia, we cannot pinpoint the precise geographic source of M293 with greater confidence.