Hart's Mill is a former flour mill complex located on a bend in the Port River, in the north-western corner of Port Adelaide, South Australia.

Its significance is described as follows:[4]Built c. 1889, this substantial mill building is associated with the development of the wheat industry in South Australia in the latter part of the 19th century and specifically with the export of flour from the state through Port Adelaide.

The Packing Shed is an uncommon surviving example of an ancillary milling industry building.

(HB Assessment Report 12/03)After his final voyage to England in 1846 John Hart settled near Port Adelaide, where he joined with H. Kent Hughes as merchants Hughes and Hart then, as Hart & Company, established large and successful flour mills.

The flour mill at Port Adelaide, now colloquially referred to as Hart's Mill, was regarded as one of the best, and "Hart's Flour" commanded the highest prices in Australia.