The film begins with an examination of the means, methods, and motives of hecklers and their effect on live performances, particularly stand up comedy.

Several male comedians suggest that female hecklers are often motivated by a desire to flirt with the performer.

Interviewees include comedians Louie Anderson, Kathy Griffin, Bobby Slayton, Patton Oswalt, Joe Rogan, Arsenio Hall, Carrot Top, Maria Bamford and George Wallace; medical doctor Drew Pinsky; writer Christopher Hitchens; political commentator Dennis Prager; producer Peter Guber; directors Joel Schumacher, George Lucas and Rob Zombie; and singer Jewel Kilcher.

Heckler also includes footage of much-derided filmmaker Uwe Boll's boxing matches with several of his most vocal critics.

[5] Joel Keller from The Huffington Post said that the film became more about "Jamie's revenge" and called it a disappointment.