After her husband's death in 1917, Lady Tree continued to act steadily for almost two decades more until towards the end of her life, in plays and some films, making her last stage appearance in 1935.
[2] She was educated at Queen's College, London, taking high honours in classics, and making her stage debut in a student production of a Greek drama.
[2] In September 1883, now billed under her married name, Mrs Beerbohm Tree, she had what The Times called "her first big hit" as Hester Gould in G. W. Godfrey's The Millionaire.
The reviewer in The Times wrote, "Another excellent impersonation is the Hester Gould of Mrs Beerbohm Tree, a lady who, although new to the stage, has manifestly the temperament and artistic sense of a fine actress.
The viperine qualities of this mysterious mischief-maker … are rendered by Mrs Beerbohm Tree with extraordinary incisiveness, and with a concentration of nervous force suggestive of Madame Sarah Bernhardt.
She appeared in Still Waters Run Deep (1916), Little Dorrit (1920), Such is the Law (1930), Wedding Rehearsal (1932), Early to Bed, The Girl from Maxim's, The Private Life of Henry VIII and Her Imaginary Lover (all in 1933), and The Man Who Could Work Miracles (1936).